Antiphospholipid Ab Panel I

Profile Code: 1811
Methodology: EIA, DVVT
CPT Codes: 86147 X 3, 85613, 86146 X 3
Specimen Requirements: 2 mL serum & 2 frozen plasma vials, 1 mL each from 2 sodium citrated tubes (blue top)
Turn Around Time: 2 – 4 Days
Special Instructions: For Lupus Anticoagulant, collect specimen in 2 sodium citrated tubes (blue top).  Mix by inverting ten times. Check for fibrin clots using wooden applicator sticks (clotted specimen is unsatisfactory).  Centrifuge immediately for 15 minutes at 1500g.  Pour into red top tube and centrifuge a second time. Do not let whole blood sit at room temperature for more than 20 minutes before centrifugation. Transfer plasma to two plastic vials, 1 mL each. Mark tube accordingly. Freeze immediately. Plasma must be separated from the red cells before freezing. Specimen must remain frozen throughout transport and shipped on dry ice (overnight if out of courier area). For convenience, separated & spun serum for other tests can be frozen and sent along with the frozen plasma. Designate on the tubes which is serum and which is plasma.
Tests in Profile: Anticardiolipin Abs (IgG, IgA & IgM Isotypes), Lupus Anticoagulant, Anti-Beta-2-Glycoprotein Ab, IgG, IgA & IgM